Mapping of Escherichia coli H27-Specific Epitope from H-Specific Polypeptides

Enteroaggregative Escherichia coli Serotype O126:H27, Israel

Enteroaggregative Escherichia coli (EAEC) is a newly diarrheagenic agent wherein several predominant serotypes are reported. We studied the association between those serotypes, as clonal indicators, and the trait of enteroaggregative adherence to host cells, tested by polymerase chain reaction. We also evaluated the clinical manifestations of infection in 17 hospitalized children by our most co...

Specific Markers of Staphylococcus aureus and Escherichia coli VBNC in Patients with Rheumatoid Arthritis

Background and purpose: Recent studies suggest that the viable but nonculturable (VBNC) bacteria could cause inflammatory diseases. The aim of this study was to detect and investigate the expression of nuc and rfbE genes in blood samples of patients with rheumatoid arthritis (RA). Materials and methods: In this experimental study, 102 blood samples were collected from patients with RA and univ...

Fed-batch Cultivation of Recombinant Escherichia coli Producing Human Interferon-γ Under Controlled Specific Growth Rate

Epitope mapping and functional characterization of monoclonal antibodies specific for the alpha subunit of Escherichia coli RNA polymerase.

The epitopes have been localized for a set of monoclonal antibodies specific for the alpha subunit of the Escherichia coli RNA polymerase. The antibodies are classified into three groups based on their epitopic assignments. Group 1, mAb 123C2, maps in the N terminus of alpha between amino acids 1 and 23; Group 2 antibodies (mAb 129C4, mAb 124D1 and mAb 121C5) map in the central region between a...

Production of specific IgY antibody to the recombinant FanC protein produced in Escherichia coli

Objective(s): Enterotoxigenic Escherichia coli (ETEC) strains are one of the primary causes of diarrhea in newborn calves and in humans, pigs, and sheep. IgY technology has been identified as a promising alternative to generating a mass amount of specific antibody for use in immunotherapy and immunodiagnostics. The purpose of this study was to produce specific antibody by egg yolk antibody (IgY...